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KMID : 0381919970270020111
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Korean Journal of Microscopy
1997 Volume.27 No. 2 p.111 ~ p.120
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Structural Analysis of Lamellar Plane in Three-Dimensional Crystal of $Ca^{2+}-ATPase$
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Cheong Gang-Won
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Abstract
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Cylinder-like crystals of $Ca^{2+}-ATPase$ provide views parallel to the lamellar plane, from which parameters of lamellar stacking can be directly measured. These parameters were measured using different preparation methods. Assuming that molecular packing is the same, data from lamellar plane could supplement those obtained by tilting large, thin plate-like crystals. However, base on data obtained .by electron microscopy and x-ray powder patterns, the plate-like crystal may have another scheme for stacking the lamellar. The projection map (h, 0, 1) from cylinder-like crystals using cryoelectron microscopy suggest the lamellar spacing can be variable.
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KEYWORD
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$Ca^{2+}-ATPase$, Cryoelectron microscopy, X-ray, Negative Stain, Image Analysis
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